外泌体BCA法测蛋白浓度的时候需要裂解吗？

LarryYang

中义 发表于 2016-9-29 12:42
测试结果相近。

那请问下，裂解后的还要离心取上清吗，像细胞一样？还是说不用离心？

中义

五仁 发表于 2016-9-24 00:08
对比效果如何？

测试结果相近。

五仁

中义 发表于 2016-9-23 17:17
直接测和裂解后测都可以，我对比过一次。

对比效果如何？

中义

直接测和裂解后测都可以，我对比过一次。

免疫小子

徐风 发表于 2016-6-15 22:13
The authors usually obtain 0.3 to 0.5µg of exosomes per 106 dendritic cells in 24 hr (Segura e ...

非常感谢啊  知道啦

徐风

     The authors usually obtain 0.3 to 0.5µg of exosomes per 106 dendritic cells in 24 hr (Segura et al., 2005).
    Given the lack of details in the literature, it is difficult to say today whether all cell types produce exosomes and at what level, but exosome production seems to be a rather general mechanism, and an average level of production of ∼0.1 µg/106 cells may be taken as a rough general estimate.