谢谢楼主回复!!我最近看到一篇用PEG的文章,它提出配PEG不加Nacl提取效果会更好呢,而且它是用的PEG 10000,楼主有试过吗?。Weng Y, Sui Z, Shan Y, Hu Y, Chen Y, Zhang L, Zhang Y. (2016) Effective isolation of exosomes with polyethylene glycol from cell culture supernatant for in-depth proteome profiling.