十年磨一剑，终于做完啦！！--投稿全记录

惜名

好吧，我承认我是标题党。。。
--------------------------------------
鄙人实验室有2个人主要做exo，一个是师兄（其实和我一届，但年龄比我大很多），一个是我。
师兄平时要上临床，因此很忙，做的是DC-exo-Cardiomyocytes，未涉及很多机制，匆匆做了点现象，就投稿了。
被拒了。。。
其中一条拒稿意见是这样的：
The method of exosome isolation - ExoQuick is a well accepted method of exosome isolation. The yield is high, but the specificity is low. This paper (Lobb RJ Optimized exosome isolation protocol for cell culture supernatant and human plasma, J Extra Vesc, 2015) nicely sums up the problem: "In conclusion, both ExoQuickTM and Exo-spinTM have significantly more non-exosomal protein contamination as evidenced by the particle/protein ratio combined with increased exosome marker expression in qEV and density gradient isolations."  This is borne out by your suspiciously high yield of protein from exosomes 10+ ug.  Therefore, a number of non-exosomes may also be present in the tail-vein injections resulting in signaling from these activated dendritic cells.  I encourage you to make note of this methodologic limitation in your conclusion.
用kit的童鞋们，都要小心啦。。。
----------------------------------------
鄙人也基本做完了，正在整理数据写文章。
本着21世纪的“互联网分享精神”，打算在下次小聚时与大家分享我所有的结果。

OK，你们终于发现了，这其实是一个广告贴！！！
欢迎来参加“魔都外泌体论坛第三次线下聚会”
时间初步定于十一之后的第一个周末，地点在上海·二军大。

惜名

yy8651 发表于 2015-9-6 20:31
哎呀呀，投的哪个杂志啊？意思就是kit不行呗？

投的JMCC